Part:BBa_K419006

alkB1 with rbs

alkB1 is alkane 1-monooxygenase from alkB1GHJ operon of a hydrocarbone-degrating marine bacterium, Alcanivorax Borkumensis SK2. When A. Borkumensis SK2 doing oil degradation, alkB1 turn long chain alkane of crude oil into alkanol. We construct alkB1 with RBS (BBa_B0034). This part is provided by project: Poseidon- save the world.

NCBI GENE ID: 4213396

Note: alkB1 gene have 2 EcoRI sites, do not digest DNA with EcoRI. Digest alkB1 with SpeI and PstI is OK.

Reference

Schneiker, et al. Genome sequence of the ubiquitous hydrocarbon-degrading marine bacterium Alcanivorax borkumensis. Nature Biotechnology 24 (2006).

MM Yakimov, et al. Obligate oil-degrading marine bacteria. Curr Opin Biotechnol (2007 Jun).

JS Sabirova, et al. Proteomic Insights into Metabolic Adaptation in Alcanivorax borkumensis Induced by Alkane Utilization.Journal of Bacteriology (2006 Jun).

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 918
  Illegal EcoRI site found at 1168
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 918
  Illegal EcoRI site found at 1168
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 918
  Illegal EcoRI site found at 1168
  Illegal BglII site found at 994
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 918
  Illegal EcoRI site found at 1168
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 918
  Illegal EcoRI site found at 1168
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI.rc site found at 661